Uji Aktivitas Antibakteri Daun Gandarusa Justicia Gendarussa Burm f Terhadap Bakteri Escherichia coli dan Staphylococcus aureus
نویسندگان
چکیده
Perkembangan dalam pengobatan tradisional semakin marak dan banyak digunakan untuk berbagai penyakit terutama antibakteri. Tujuan penelitian ini mengatahui aktivitas antibakteri Daun Gandarusa (Justicia Gendarussa Burm.f) terhadap bakteri Escherichia coli Staphylococcus aureus. Pada bersifat eksperimental. Tahap diawali dengan ekstraksi pelarut metanol fraksinasi N-heksan pada sehingga menghasilkan 2 fraksi yaitu Nheksan, uji dilakukan metode disc difusi untukpengukuran daya hambat konsentrasi 1000 ppm; 500 250 125 62,5 ppm serta kontrol negatif menggunakan aquades positif ampicillin. Selanjutnya, pengujian makrodilusi untukpenentuan nilai KHM KBM. Senyawa yang memiliki tinggi diuji Gas Chromatography-Mass Spectrometry (GC-MS). Hasil GC-MS kemudian secara In Silico mengetahui senyawa tersebut berikatan protein digunakan. in vitro silico baik sebagai Secara dapat dilihat dari zona hambat/zona bening terbentuk melalui Untuk berdasarkan kekuatan interaksi terjadi.
منابع مشابه
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ژورنال
عنوان ژورنال: Sainsbertek Jurnal Ilmiah Sains & Teknologi
سال: 2022
ISSN: ['2797-183X', '2797-1244']
DOI: https://doi.org/10.33479/sb.v3i1.183